Study on preparation and identification of monoclonal antibodies immunized with H5N1 influenza virus M1 protein.
- Author:
Ai-jun CHEN
1
;
Li-hong YAO
;
Jian-qiang GUO
;
Yi XU
;
Xiao-yu LIU
;
Run-qing JIA
;
Zhi-qing ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; analysis; immunology; Antibodies, Viral; analysis; immunology; Cell Line; Cross Reactions; Dogs; Humans; Influenza A Virus, H5N1 Subtype; genetics; immunology; Influenza, Human; immunology; virology; Mice; Mice, Inbred BALB C; Viral Matrix Proteins; genetics; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(6):412-414
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare monoclonal antibodies specific for M1 protein of H5N1 subtype human influenza virus, this work may provide new tool in rapid diagnosis and study of type A influenza virus.
METHODSBALB/c mice were immunized with purified recombinant H5N1 (A/Anhui/1/2005)/M1 protein expressed in E. coli. Spleen cells of the immunized mice were fused with sp2/0 cells to produce hybridoma cell lines. ELISA was performed to identify the monoclonal antibody against M1 protein of H5N1. Immunofluorescence assay (IFA) were applied to identify the specificity of these antibodies.
RESULTSThree hybridoma cell lines steadily secreting anti-H5N1/M1 McAb were obtained, and their cross reactivity was confirmed by cross-reaction test and IFA.
CONCLUSIONMonoclonal antibodies immunized with H5N1 subtype influenza virus M1 protein are cross-reactive, which can be used to detect different subtype of influenze virus type A.
