Comparison of the immnunogenicity of rAAV2/1 and rAd5 rad5 expressing HIV-1 gag.
- Author:
Shuang-qing YU
1
;
Xia FENG
;
Hong-mei LIU
;
Hai-ru YANG
;
Hong-xia LI
;
Yi ZENG
Author Information
- Publication Type:Journal Article
- MeSH: AIDS Vaccines; administration & dosage; genetics; immunology; Adenoviridae; genetics; metabolism; Animals; Dependovirus; genetics; metabolism; Female; Gene Expression; HIV Antibodies; blood; immunology; HIV Infections; immunology; prevention & control; virology; HIV-1; genetics; immunology; Humans; Mice; Mice, Inbred BALB C; Random Allocation; gag Gene Products, Human Immunodeficiency Virus; administration & dosage; genetics; immunology
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(6):421-423
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the immunogenicity of rAAV2/1 and rAd5 expressing HIV-1 gag in BALB/c mice.
METHODSBALB/c mice were immunized with rAAV2/1-gag or rAd5-gag once or twice. HIV-1 specific cellular immune responses were analyzed by in vivo CTL and intracellular cytokine staining assays. HIV-1 Gag specific antibodies were tested by ELISA.
RESULTSMice immunized with rAd5-gag once induced stronger Gag specific cellular immune responses and similar level of Gag specific antibody compared with rAAV2/1-gag. Mice immunized with rAd5-gag reached the peak immune responses more rapidly than rAAV2/1-gag. However, mice immunized with rAAV2/1-gag twice elicited better Gag specific IgG.
CONCLUSIONrAd5-gag induced strong HIV-1 specific cellular and antibody responses, and rAAV2/1-gag induced high level of HIV-1 specific IgG and moderate cellular immune responses.
