Comparison of the immunogenicity of rAAV2/1 and rAd5 expressing HIV-1 gag
10.3760/cma.j.issn.1003-9279.2009.06.007
- VernacularTitle:表达HIV-1 gag基因的重组AAV2/1和Ad5疫苗免疫原性比较
- Author:
Shuang-Qing YU
1
;
Xia FENG
;
Hong-Mei LIU
;
Hai-Ru YANG
;
Hong-Xia LI
;
Yi ZENG
Author Information
1. 中国疾病预防控制中心
- Keywords:
HIV-1;
Parvoviridae;
Adenoviridae;
AIDS Vaccines
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(6):421-423
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the immunogenicity of rAAV2/1 and rAd5 expressing HIV-1 gag in BALB/c mice.Methods BALB/c mice wcre immunized with rAAV2/1-gag or rAd5-gag once or twice.HIV-1 specifc cellular immune responses were analyzed by in vivo CTL and intracellular cymkine staining assays.HIV-1 Gag specifc antibodies were tested by ELISA.Results Mice immunized with rAd5-gag once induced stronger Gag specific cellular immune responses and similar level of Gag specific antibody compared with rAAV2/1-gag.Mice immunized with rAd5-gag reached the peak immune responses more rapidly than rAAV2/1-gag.However,mice immunized with rAAV2/1-gag twice elicited better Gag specific IgG.Conclusion rAd5-gag induced strong HIV-1 specifc cellular and antibody responses,and rAAV2/1-gag induced high level of HIV-1 specific IgG and moderate cellular immune responses.
