Expression of partial S gene of Hantavirus Z10 in Pichia pastoris.
- Author:
Han-ping ZHU
1
;
Ping-ping YAO
;
Xiao-zhao DENG
;
Fang XU
;
Rong-hui XIE
;
Zhi-yong ZHU
;
Yun ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Gene Expression; Hantavirus; genetics; metabolism; Nucleocapsid Proteins; genetics; metabolism; Pichia; genetics; metabolism
- From: Chinese Journal of Experimental and Clinical Virology 2009;23(6):434-436
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe purpose of this study is to express partial S gene of Hantavirus Z10.
METHODSThe 300 bp S gene of Z10 strain was synthesized by using a successive PCR method for the optimal expression in Pichia pastoris and subcloned into pMD19-T. The SP300 gene was constructed into pPICZaA and sequenced. The recombinant pPICZaA-SP300 and pPICZaA-S300 was transformed into Pichia with LiCI.
RESULTSThe recombination Pichia were cultivate, and expressed the SP300 or S300 gene induced in Pichia by methanol.
CONCLUSIONThe nucleocapsid secreted from the Pichia can be detected by ELISA and WesternBlot.
