Expression of partial S gene of Hantavirus Z10 in pichia pastoris

VernacularTitle:汉坦病毒部分S基因在毕赤酵母中的分泌表达
Author: Han-Ping ZHU ¹ ; Ping-Ping YAO ; Xiao-Zhao DENG ; Fang XU ; Rong-Hui XIE ; Zhi-Yong ZHU ; Yun ZHANG
Author Information

1. 浙江省疾病预防控制中心
Keywords: Hantavirus; Grenes; Gene expression; Pichia
From: Chinese Journal of Experimental and Clinical Virology 2009;23(6):434-436
CountryChina
Language:Chinese
Abstract: Objective The purpose of this study is to express partial S gene of Hantavims Z10.Methods The 300 bp S gene of Z10 strain was synthesized by using a successive PCR method for the optimal expression in Pichia pastoris and subcloned into pMD19-T.The SP300 gene was constructed into pPICZaA and sequenced.The recombinant pPICZaA-SP300 and pPICZaA-S300 was transformed into Pichia with LiCl. ResultsThe recombination Pichia were cultivate,and expressed the SP300 or S300 gene induced in Pichia by methanol.Conclusion The nucleocapsid secreted from the Pichia can be detected by ELISA and WestemBlot.