Study on immunogenicity of a recombinant adenovirus vaccine containing neuraminidase gene of H5N1 influenza virus(A/Anhui/1/2005)in mice
10.3760/cma.j.issn.1003-9279.2009.06.016
- VernacularTitle:含有H5N1 NA基因重组腺病毒疫苗在小鼠体内的免疫效果研究
- Author:
Jing MA
1
;
Xiao-Guang ZHANG
;
Kui-Biao LI
;
Xiao-Mei ZHANG
;
Min WANG
;
Tian BAI
;
Liang YANG
;
Hong XU
;
Yue-Long SHU
;
Yi ZENG
Author Information
1. 北京工业大学
- Keywords:
Influenza A virus;
Neuraminidase;
Adenoviruses;
human;
Immunity;
active
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(6):449-451
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate immunity of a recombinant adenovirus vaccine(rAdV)containing codon-modified neuraminidase(Mod.NA)gene of H5N1 influenza virus in BALB/c mice and to screen for appropriate dose.Methods BALB/c mice were immunized with the rAdV-Mod.NA vaccine intramuscularly twice (double injection at 0 and 4~(th) week)in three groups,low dosage(10~5 TCI_(50) per dose),medium dosage(10~7 TCID_(50) per dose)and high dosage(10~9 TCID_(50) per dose).The effect of humoral and cell-mediated immunity were analysod at 5~th week.Results ①The rAdV-Mod.NA vaccine eould elicit both humoral and cell-mediated robust NA specific immunity in mice by neuraminidase inhibitor assay and IFN-γ ELISpot assay;②10~7 TCID_(50) per dose Was the appropriate dose;③ Peptide NA_(109-124):CRTFFLTQGALLNDKH and peptide NA_(182-199):AVAVLKYNGIITDTIKSW were the dominant epitopes for neuraminidase-immunized BALB/c mice,which was screened out from the whole length of neuraminidase of an H5N1 virus,A/Anhui/1/2005.Conclusion The recombinant adenovirus NA could induce specific humoral and cellular immune responses in BALB/c after immunization,which suggest rAdV-Mod.NA vaccine was a potential vaccine candidate against H5N1 influenza and worthy of further investigation.
