Human papillomavirus type 16 E5 sequence evolution analysis

VernacularTitle:人乳头瘤病毒16型E5序列进化分析
Author: Min HAO ¹ ; Xiao-Zhou WU ; Xiao-Li WANG ; Mei-Lu BIAN ; Hong-Tu LIU
Author Information

1. 北京协和医学院
Keywords: Papillomavirns; human; Variantion(Genefics); Sequence analysis
From: Chinese Journal of Experimental and Clinical Virology 2009;23(6):452-454
CountryChina
Language:Chinese
Abstract: Objective To investigate the distribution of HPV 16 variants in Hart women patients without Cervical intraepithelial neoplagia in the diagnosis and treatment center for cervical disease.department of Obstetrics and Gynecology in China-Japan friendship hospital with HPV 16 E5 sequence phylogenetic analysis.Methods PCR amplification of HPV 16 E5 sequences and sequenced.The association between variations types and different cervical lesions was analyzed.Results In this research,We first found that variant classification based on HPV 16 E5 DNA sequence(236 bp)alone had hish rate of accuracy.In addition,for the first time,our research revealed that single-4075T can distinguished the As variant from all other variants.Conclusion If E5 sequence wag used for phylogenetic analysis,it will gready reduce the experimental costs and improve efficiency and cost-effectiveness.For the first time.our research revealed that single 4075T can distinguished the As variant from all other variants.