Bioinformatics analysis of mosquito densovirus nostructure protein NS1
10.3760/cma.j.issn.1003-9279.2009.06.020
- VernacularTitle:蚊浓核病毒非结构蛋白1的生物信息学分析
- Author:
Yun-Qiao DONG
1
;
Wen-Li MA
;
Jin-Bao GU
;
Wen-Ling ZHENG
Author Information
1. 南方医科大学
- Keywords:
Densovirus;
Viral nonstructural proteins;
Computational biology
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(6):461-463
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze and predict the structure and function of mosquito densovirus(MDV)nostmetual proteinl(NSI).Methods Using different bioinformafics software,the EXPASY pmtparam tool,ClustalX1.83,Bioedit,MEGA3.1,ScanProsite,and Motifscan,respectively to comparatively analyze and predict the physic-chemical parameters,homology,evolutionary relation,secondary structure and main functional motifs of NS1.Results MDV NS1 protein wag a unstable hydrophilic protein and the amino acid sequence wag highly conserved which had a relatively closer evolutionary distance with infectious hypodermal and hematopoietic necrosis virus(IHHNV).MDV NS1 hag a specific domain of superfamily 3 helicage of small DNA viruses.This domain contains the NTP-binding region with a metal ion-dependent ATPase activity.A virus replication roller rolling-circle replication(RCR)initiation domain wag found near the N terminal of this protein.This protien has the biological function of single stranded incision enzyme.Conclusion The bioinformatics prediction results suggest that MDV NS1 protein phys a key role in viral replication,packaging,and the other stages of viral life.
