Evaluation of genotyping of Human Papillomavirus by a new DNA Liquid Chip based on Luminex XMAP System
10.3760/cma.j.issn.1003-9279.2009.06.025
- VernacularTitle:Luminex XMAP液相芯片技术检测人乳头瘤病毒基因型的应用评价
- Author:
Xiao-Mei DENG
1
;
Min LIU
;
Gui-Xi ZHENG
;
Chuan-Xin WANG
;
Jian ZHANG
;
Wei LI
Author Information
1. 山东大学齐鲁医院
- Keywords:
Popillomavims;
human;
Cervix neoplasms;
Cervical intraepithelial neoplasia;
Genotype;
Liquid chip
- From:
Chinese Journal of Experimental and Clinical Virology
2009;23(6):476-478
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the diagnostic value of Luminex XMAP liquid chip for HPv detection.Methods Detect HPV DNA with the liquid chip and HC Ⅱ in 264 cases,including 231 of cervical cancer and 33of cervical scrapes.ne accordance of two methods were compared and DNA sequencing was performed in conflicting samples and single(infection samples.Results There is an excellent agreement between the methods.The overall incidence of HPV was 82.95%,the most common genotypes were HPV 16,52,58,18,11,31,6,39,33,56,70.Among all the positive types,117 were single type and 102 were multiple types,and 87.43% were high-risk HPV genotypes and 12.57% were low-risk genotypes.Based on the criteria of histology and pathology.the sensitivity,specificity,positive and negative-predictive value of HPV liquid chip and HC Ⅱ for detecting all cases of CIN Ⅱ,Ⅲand carcinomas were respectively 93.07%,87.88%,98.17%,64.44% and 94.81%,87.88%,98.21%,70.73%.Conclusion The common types of HPV infection are 16,52,58,18,11,6,56and 31.Multiplex HPV genotyping by Luminex XMAP liquid chip appears to be highly suitable for diagnostic screening and large-scale epidemiological studies.
