A nested multiplex PCR assay for rapid subtyping the prevailing HIV-1 strains in Guangxi

VernacularTitle:应用多重巢式PCR法快速鉴定广西HIV-1主要亚型
Author: Qi-Jian SU ¹ ; Ping ZHOU ; Zhi-You BI ; Xin XIAO ; Shu-Zhi WU ; Ping CEN ; Wei DENG ; Yi-Ming SHAO ; Hao LIANG
Author Information

1. 广西医科大学艾滋病研究中心
Keywords: Polymerase chain reaction; HIV-1; Genotype
From: Chinese Journal of Experimental and Clinical Virology 2009;23(6):482-484
CountryChina
Language:Chinese
Abstract: Objective To establish a rapid nested multiplex PCR assay for subtyping HIV-1 CRF01_AE,CRF07_BC,CRF08_BC,B,and C strains prevailing in Guangxi.Method Subtype-specific primers were designed for these subtypes based on their gag sequences.The subtypes of HIV-1 samples from Gmmgxi were determined by nested multiplex PCR and DNA sequencing and phylogenetic analysis,respectively,and then the sensitivity and the specificity of nested multiplex PCR were calcuhted.Results Nested multiplex PCR could correctly classify the 5 known-subtype samples,and were not reactive to all HIV-negative samples.Of the 72 HIV-positive samples,66 were correctly identified as CRFO1_AE,CRF07_BC,CRF08_BC,and B by this assay,giving a sensitivity of 91.7%(66/72),and a specificity of 100%.Conclusion This assay is a simple,fast,and cost-effective subtyping method for HIV-1 CRF01_AE,CRF07_BC,CRF08_BC,and B strains prevailing in Guangxi.