Effect of deltamethrin on benzyloxyresorufin O-dealkylatase activity and CYP2B1/2B2 protein level in rat brain.
- Author:
Liegang LIU
1
;
Hong YAN
;
Nian SHI
;
Yugu LIU
;
Ping YAO
;
Xuefeng YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aryl Hydrocarbon Hydroxylases; antagonists & inhibitors; biosynthesis; Blotting, Western; Brain; drug effects; enzymology; Cytochrome P-450 CYP2B1; antagonists & inhibitors; biosynthesis; Enzyme Inhibitors; toxicity; Insecticides; toxicity; Nitriles; toxicity; Pyrethrins; toxicity; Rats; Steroid Hydroxylases; antagonists & inhibitors; biosynthesis
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2002;20(6):422-424
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the effect of deltamethrin (DM) on benzyloxyresorufin O-dealkylatase (BROD) activity and the expression of CYP2B1/2B2 in rat brain.
METHODSBROD activity was determined by fluorophotometry under the treatment of DM in vivo and vitro. Western-blot analysis was used to detect the expression of CYP2B1/2B2.
RESULTSIn vivo, DM could markedly inhibit BROD activity in rat brain microsome after rats had been treated with DM (12.5 mg.kg-1.d-1, i.p.) for 5 days. The inhibitory rate in whole brain, cerebral cortex and cerebellum were 26.7%, 23.8% and 33.3% respectively(P < 0.05). However, in vitro, under the concentration of 2 x 10(-8)-2 x 10(-4) mol/L of DM, there was no obvious change of BROD activity in rat brain. Moreover, Western-blot analysis indicated that DM could significantly reduce the expression of CYP2B1/2B2 in vivo, the inhibitory rate of protein synthesis was 42.6%.
CONCLUSIONDM could inhibit BROD activity in rat brain and this effect may be related to the reduction of CYP2B1/2B2 protein synthesis.
