Changes of phosphorylation states of Cx43 protein in CCL-64 cells induced by SiO2.
- Author:
Weiwei WU
1
;
Guogen MAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Communication; drug effects; Cell Line; Connexin 43; metabolism; Gap Junctions; drug effects; Lung; drug effects; metabolism; Mink; Phosphorylation; Silicon Dioxide; toxicity; Tetradecanoylphorbol Acetate; pharmacology
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2002;20(6):458-460
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether the cellular gap junctional communication(GJIC) down-regulation in alveolar epithelial cells (CCL-64 cells) induced by silica-stimulated pulmonary alveolar macrophages (PAM) supernatant is related with the phosphorylation states of connexin 43(Cx43) protein.
METHODWestern-blot analysis was used to identify phosphorylated Cx43 species.
RESULTSWestern-blot analyses of SiO2- and phorbol 12-myristate 13-acetate(TPA)-treated CCL-64 cells showed the same phosphorylation states of Cx43 as the control group. There were no Cx43 protein in nucleus of CCL-64 cells.
CONCLUSIONThe inhibition on GJIC induced by SiO2 and TPA in CCL-64 cells may not be brought about by altering the phosphorylation states of Cx43.
