The advancement of AFLP technology.
- Author:
Tao LI
1
Author Information
1. Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou, China. yzulitao@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Amplified Fragment Length Polymorphism Analysis;
methods;
Microsatellite Repeats;
Polymorphism, Single Nucleotide;
Quantitative Trait Loci
- From:
Chinese Journal of Biotechnology
2006;22(5):861-865
- CountryChina
- Language:Chinese
-
Abstract:
AFLP technology has been widely used in molecular biology due to its integration of several advantages of high throughput, high efficiency and requiring no sequence information, etc. Great changes have been achieved in recent years in AFLP-related technologies and platforms. There are several AFLP-expanded technologies available. These improved technologies are capable of distinguishing the heterozygote from the homozygote and of converting any AFLP band of interest, without much effort, into locus-specific markers, which can be deployed for massive locus detection and for gene isolation. This review focuses on these favorable changes from conventional AFLP technology into more effective and more practicable AFLP-related ones. Understanding these advancements and AFLP-expanded technologies will facilitate the achievement of our research goals.
