Regeneration of transgenic Lespedeza thunbergii expressing levansucrase gene (Sac B) from Bacillus subtilis.
- Author:
Jin-You DU
1
;
Xiao-Yang CHEN
;
Gui-Rong ZHANG
;
Wei LI
;
Dong-Nan HU
;
Zan-Min HU
Author Information
1. Hebei Normal University of Science and Technology, Qinhuangdao 066600, China.
- Publication Type:Journal Article
- MeSH:
Bacillus subtilis;
enzymology;
genetics;
Carbohydrate Metabolism;
Carbohydrates;
chemistry;
Hexosyltransferases;
genetics;
Lespedeza;
drug effects;
genetics;
growth & development;
metabolism;
Plants, Genetically Modified;
Reverse Transcriptase Polymerase Chain Reaction;
Sodium Chloride;
pharmacology;
Solubility;
Stress, Physiological;
drug effects;
Transformation, Genetic;
Transgenes;
genetics
- From:
Chinese Journal of Biotechnology
2006;22(6):940-944
- CountryChina
- Language:Chinese
-
Abstract:
The Bacillus subtilis Sac B gene with the vacuolar targeting signal sequence driven by 35S promotor was transferred into Lespedeza thunbergii by Agrobacterium mediated method. Total 62 Kan-resistant plants were obtained, of which 5 plants were proved to be transgenic plants. The transgenic plants were characterized by PCR amplification, PCR-Southern hybridization and RT-PCR. The physiological assay results showed that the transgenic plants were more tolerant to stress than the controls under the condition of 200mmol/L NaCl and 5% PEG, respectively, and that the content of soluble sugar in trnsgenic plants was significantly higher than that of controls in the period of tests (5-15 days) under salt and PEG stress.
