Expression of porcine beta-defensin 1 gene in Pichia pastoris.
- Author:
Li-Hua JIANG
1
;
Hai-Rong LU
;
De-Xin HUANG
;
Jun-Bo YI
;
Ling-Yun LI
;
Feng LIN
Author Information
1. National Engineering Center for Biotechnology, Shenzhen 518057, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Anti-Bacterial Agents;
biosynthesis;
isolation & purification;
pharmacology;
Cloning, Molecular;
Gene Expression;
Genetic Vectors;
genetics;
Pichia;
genetics;
Protein Engineering;
Staphylococcus aureus;
drug effects;
Swine;
genetics;
beta-Defensins;
biosynthesis;
genetics;
isolation & purification;
pharmacology
- From:Chinese Journal of Biotechnology
2006;22(6):1036-1039
- CountryChina
- Language:Chinese
-
Abstract:
PBD-1 is an antibacterial peptide that plays an important role in defence system of porcine. To produce PBD-1 with bioactivity in Pichia pastoris, according to published amino acid sequence of porcine beta-defensin 1(PBD-1) and the partiality codon of yeast, the PBD-1 gene was synthesized by PCR and cloned into pPIC9K to construct the recombinant expression vector pPIC9K-PBD-1, the obtained recombinant plasmid was linearized by Sal I, and then transformed into SMD1168 by electroporation. Under the control of the promoter AOX1, an approximately 4.5 kD PBD-1 peptide was expressed. Antibacterial activity assay shows that the PBD-1 has the antibacterial activity on Staphylococcus aureus. This is the first secreted expression of porcine beta-defensin 1 gene in Pichia pastoris.
