Progress of oligosaccharides biosynthesis in recombinant Escherichia coli.
- Author:
Da-Wei ZHANG
1
;
Peng WANG
;
Qing-Sheng QI
Author Information
1. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
- Publication Type:Journal Article
- MeSH:
Bacteria;
enzymology;
genetics;
Bacterial Proteins;
genetics;
metabolism;
Carbohydrate Sequence;
Escherichia coli;
genetics;
metabolism;
Genetic Engineering;
methods;
trends;
Glycosyltransferases;
genetics;
metabolism;
Industrial Microbiology;
methods;
trends;
Molecular Sequence Data;
Oligosaccharides;
biosynthesis
- From:
Chinese Journal of Biotechnology
2007;23(1):16-20
- CountryChina
- Language:Chinese
-
Abstract:
As more bioactivities of oligosaccharides have been elucidated, researches on biosynthesis of oligosaccharides have drawn more concerns in Glycobiology. A lot of enzymatic methods for the synthesis of oligosacchrides have been developed employing recombinant E. coli expressed glycosyltranferase or synthase of nucleotide-sugar. This review focuses on the recent progress in the production of oligosaccharides using bacteria especially by genetically engineered bacteria. The key point concering the oligosaccharides biosynthesis in recombinant E. coli, such as enzyme expression, NDP-sugar provision and biosynthesis pathway, was discussed.
