Study on kinetics of two-stage cultivation of protocorm-like bodies from Dendrobium huoshanense for cell growth and synthesis of polysaccharides.
- Author:
Ming WEI
1
;
Shao-Tong JIANG
;
Jian-Ping LUO
Author Information
1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
- Publication Type:Journal Article
- MeSH:
Algorithms;
Biomass;
Cell Culture Techniques;
Cell Proliferation;
Cells, Cultured;
Dendrobium;
cytology;
metabolism;
Kinetics;
Models, Biological;
Plant Stems;
cytology;
metabolism;
Polysaccharides;
biosynthesis;
Time Factors
- From:
Chinese Journal of Biotechnology
2007;23(1):79-84
- CountryChina
- Language:Chinese
-
Abstract:
Phosphate is an effective factor in the regulation of cell growth and accumulation of polysaccharides. The method for higher polysaccharide production has been obtained through feeding cultivation. The two-stage cultivation was proposed according to the characteristic of cell growth and the accumulation of polysaccharides in the suspension cultures of protocorm-like bodies from Dendrobium huoshanense. A kinetic model was developed to describe the cultivation process. The results indicate that the production of biomass and polysaccharides increases from 28.7g DW/L and 1.86g/L to 44.2g DW/L and 5.22g/L, respectively, and the content of polysaccharides increases from 6.4% to 11.9% . With the evaluated model parameters, the model appears to provide a description for the cultivation process.
