Isolation and characterization of H2-producing strains Enterobacter sp. and Clostridium sp.
- Author:
Xiao-Peng ZHI
1
;
Qing-Feng LIU
;
Xiao-Bing WU
;
Hui-Juan XU
;
Min-Nan LONG
Author Information
1. School of Life Sciences, Bio-Energy Center, Xiamen University, Xiamen 361005, China.
- Publication Type:Journal Article
- MeSH:
Clostridium;
isolation & purification;
metabolism;
ultrastructure;
Enterobacter;
isolation & purification;
metabolism;
ultrastructure;
Fermentation;
Glucose;
metabolism;
Hydrogen;
metabolism;
Hydrogen-Ion Concentration;
Maltose;
metabolism;
Microscopy, Electron, Transmission;
Polymerase Chain Reaction;
RNA, Ribosomal, 16S;
genetics;
Species Specificity;
Sucrose;
metabolism;
Temperature
- From:
Chinese Journal of Biotechnology
2007;23(1):152-156
- CountryChina
- Language:Chinese
-
Abstract:
Two hydrogen-producing bacterial strains were newly isolated and identified as Enterobacter sp. Z-16 and Clostridium sp. C-32 by 16S rDNA sequence analysis. Various parameters for hydrogen production, including substrates, initial pH and temperature, have been studied. The optimum condition for hydrogen production of strain Z-16 were achieved as: initial pH7.0, temperature 35 degrees C , sucrose as the favorite substrate. In comparison, The optimum condition for hydrogen production of strain C-32 were obtained as: initial pH8.0, temperature 35 degrees C , maltose as the favorite substrate . Under batch fermentative hydrogen production conditions, the maximal hydrogen conversion rate for strain Z-16 and strain C-32 were 2.68 mol H2/mol sucrose and 2.71mol H2/mol maltose, respectively. Using glucose as substrate, the hydrogen conversion rate of strain Z-16 and strain C-32 were 2.35 and 2.48 mol H2/mol glucose, respectively. This research suggest a good application potential of strain Z-16 and C-32 in the future biological hydrogen production.
