A rapid method for preparation of plasmid DNA for screening recombinant clones.
- Author:
Xu-Dong GUO
1
;
Shu-Yan MAO
;
Dong-Xia HOU
;
Shorgan BOU
Author Information
1. The Key Laboratory for Mammalian Reproductive Biology and Biotechnology, Ministry of Education, Inner Mongolia University, Hohhot 010021, China.
- Publication Type:Journal Article
- MeSH:
Centrifugation, Density Gradient;
Chloroform;
chemistry;
Cloning, Molecular;
methods;
DNA, Bacterial;
genetics;
isolation & purification;
metabolism;
Deoxyribonuclease HindIII;
metabolism;
Electrophoresis;
Escherichia coli;
genetics;
Pentanols;
chemistry;
Phenol;
chemistry;
Plasmids;
chemistry;
genetics;
Reproducibility of Results;
Time Factors;
Water;
chemistry
- From:
Chinese Journal of Biotechnology
2007;23(1):176-178
- CountryChina
- Language:Chinese
-
Abstract:
A simple and rapid method for preparation of plasmid DNA from overnight incubation was introduced. It does not require any additional reagents; the incubation mixture containing recombinant plasmid DNA was just mixed with H2O and phenol/chloroform/isoamyl alcohol in certain ratio. After vortexing and spinning of the mixture, the supernatant could be directly loaded onto agarose gel and analyzed using electrophoresis. The whole preparation requires only 3-5 minutes. So to quickly screen recombinant clones, this method is better compared with traditional methods.
