Survivin antisense oligonucleotide induces human Hep-2 cell apoptosis.
- Author:
Yang LIU
1
;
Lin-lin YU
;
Yong-hua CUI
;
Yu-xin JI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Apoptosis Regulatory Proteins; pharmacology; Caspase 3; metabolism; Cell Proliferation; drug effects; Hep G2 Cells; Humans; Inhibitor of Apoptosis Proteins; Microtubule-Associated Proteins; genetics; pharmacology; Oligonucleotides, Antisense; pharmacology; RNA, Messenger; genetics
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(8):571-574
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESurvivin highly overexpresses in the most of human tumors, and it may play an important role in the development of tumor. The aim of this study was to explore the effects of survivin antisense oligonucleotide (ASODN) on the proliferation and the apoptosis of human Hep-2 cell.
METHODSHep-2 cells were transfected with survivin ASODN mediated by lipofectamine, MTT [3-(4,5-dimethylthiazol-2-yl)-2, 5 diphenyl tetrazolium bromide] method was used to observe the cell growth inhibitory rate, the expressions of survivin mRNA and protein were detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot respectively. Flow cytometry was used to examine cell apoptosis rate. Kinase activity test was used to detect the changes of caspase-3 activity.
RESULTSSurvivin ASODN obviously inhibited the cell growth of Hep-2 cells after transfection. After transfected with survivin ASODN the expressions of survivin mRNA and protein of Hep-2 cells were down-regulated, and apoptosis rate was significantly increased. The activity of caspase-3 increased highly in Hep-2 cells transfected with survivin ASODN, which showed time-dependent.
CONCLUSIONSSurvivin ASODN could inhibit the proliferation of Hep-2 cell and induced apoptosis through down-regulating the the expressions of survivin mRNA and protein.
