Effect of metformin on acute promyelocytic leukemia cell line NB4 and its mechanism.
- Author:
Lei HUAI
1
;
Cui-Cui WANG
;
Cui-Ping ZHANG
;
Qi-Hui LI
;
Yi-Rui CHEN
;
Yu-Jiao JIA
;
Min WANG
;
Jian-Xiang WANG
Author Information
1. State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Adhesion;
drug effects;
Cell Line, Tumor;
Extracellular Signal-Regulated MAP Kinases;
metabolism;
Humans;
Leukemia, Promyelocytic, Acute;
metabolism;
pathology;
MAP Kinase Signaling System;
drug effects;
Metformin;
pharmacology;
Mitogen-Activated Protein Kinase Kinases;
metabolism;
Phosphorylation
- From:
Journal of Experimental Hematology
2012;20(6):1322-1326
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to investigate the effect and molecular mechanism of metformin (Met) on biological characteristics of acute promyelocytic leukemia (APL) cell line NB4. NB4 cells were treated with various concentrations of Met for different time, MTT method was used to detect cell proliferation, the alteration of cell apoptosis was analyzed by flow cytometry, and the change of cell adhesion ability was examined by cell adhesion assay. NB4 cells were pretreated with U0126, a specific inhibitor for extracellular signal-regulated kinase (ERK) phosphorylation, ERK phosphorylation was assessed by Western blot analysis, apoptosis and cell adhesion ability were evaluated by flow cytometry and cell adhesion test respectively. The results showed that Met could inhibit the cell proliferation, induce the cell apoptosis and increase the ability of cell adhesion. The pretreatment of NB4 cells with 5 µmol/L U0126 could effectively inhibit the phosphorylation of ERK, and reduce cell apoptosis and adhesion induced by 5 mmol/L Met. It is concluded that Met can inhibit the proliferation and promote the apoptosis and adhesion of NB4 cells. MEK/ERK signaling pathway may be one of the molecular mechanisms of metformin on NB4 cells.
