Effects of cucurmosin combined with common chemotherapeutics on proliferation and apoptosis of NB4 cells.
- Author:
Jie-Ming XLE
1
;
Mei LIU
;
Ting-Bo LIU
;
Ming-Huang CHEN
;
Ai-Qin YANG
;
Pei YANG
Author Information
1. School of Pharmacy, Fujian Medical University, Fuzhou 350004, Fujian province, China. jm_xie@hot mail.com
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Arsenicals;
pharmacology;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Drug Synergism;
Humans;
Leukemia, Promyelocytic, Acute;
pathology;
Oxides;
pharmacology;
Plant Proteins;
pharmacology;
Tretinoin;
pharmacology
- From:
Journal of Experimental Hematology
2012;20(6):1327-1331
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the proliferation inhibition and apoptosis induction of cucurmosin (CUS) combined with all trans-retinoic acid (ATRA) or arsenic trioxide (ATO) on human acute promyelocytic leukemia cell line NB4. MTT method was used to determine the proliferative inhibition of CUS combined with ATRA or ATO on NB4 cells, and flow cytometry was used to determine the apoptosis induction effect of CUS combined with ATRA or ATO on NB4 cells. Jin's formula was used to assess the synergistic effect of this combinations. The results showed that, compared with single drug, the proliferation inhibitory ratio and apoptotic ratio of CUS combined with ATRA or ATO on NB4 cells was higher than CUS, ATRA and ATO alone. The synergistic index (q) were all larger than 0.85, and the combined effects were significant at low concentrations. It is concluded that the CUS combined with ATRA or ATO synergistically increases the effects of proliferative inhibition and apoptosis induction on NB4 cells.
