Apoptosis-inducing effect of annexin A2 on multiple myeloma cells and its related mechanisms.
- Author:
Hong-Yu BAO
1
;
Jian-Ning WANG
;
Yan-Qiu HOU
;
Ming SONG
;
Liu-Bo ZHANG
;
Qing-Qi MENG
;
Chang-Geng RUAN
Author Information
1. Department of Hematology, the Second Affiliated Hospital, Nanjing Medical University, Nanjing 210011, Jiangsu Province, China. baohongyu74@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Annexin A2;
genetics;
Apoptosis;
genetics;
Cell Line, Tumor;
Humans;
Multiple Myeloma;
genetics;
pathology;
RNA, Small Interfering;
genetics
- From:
Journal of Experimental Hematology
2012;20(6):1384-1388
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the apoptosis-inducing effect of Annexin A2 gene (AnxA2) on multiple myeloma (MM) cells and its mechanisms. The human MM cell lines U266 and RPMI8226 were transfected by using siRNA targeting at AnxA2; the expressions of AnxA2 mRNA and protein in the siRNA-transfected cells were detected by real-time PCR and Western blot, respectively; the cell apoptosis was assayed by flow cytometry. The results showed that silencing AnxA2 gene by siRNA resulted in decreased expressions of AnxA2 gene and protein, increased apoptosis of U266 and RPMI8226 cell lines (P < 0.05), at the same time resulted in down-regulation of apoptosis-related gene expressions including p65NF-κB, IL-2, IL-6 (P < 0.05), and up-regulation of P53 gene expression (P < 0.05). It is concluded that the AnxA2 silence plays a promoting role in apoptosis of MM cell lines U266 and RPMI8226.
