Effect of homoharringtonine on expression of NF-κB and BCL-2 proteins in K562 cells.
10.7534/j.issn.1009-2137.2013.01.017
- Author:
Li-Lin YE
1
;
Wei-Ke CAO
;
Yu-Ye SHI
;
Zhi-Kui DENG
;
Shan-Dong TAO
;
Ping JI
;
Xian-Hua FU
;
Ming-Zhu ZONG
;
Yu-Feng LI
Author Information
1. Department of Hematology, Nanjing Medical University, Huaian, Jiangsu Province, China.
- Publication Type:Journal Article
- MeSH:
Flow Cytometry;
Harringtonines;
pharmacology;
Humans;
K562 Cells;
NF-kappa B;
metabolism;
Proto-Oncogene Proteins c-bcl-2;
metabolism
- From:
Journal of Experimental Hematology
2013;21(1):78-81
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of homoharringtonine (HHT) on K562 cell proliferation, apoptosis and expression of BCL-2 and NF-κB proteins. The cells proliferation was assayed with MTT method, the cell apoptosis, cell cycle and BCL-2 expression were analyzed with flow cytometry, NF-κB protein expression was detected with Western blot. The results showed that HHT concentration-dependently inhibited proliferation of K562 cells, the IC50 at 48 h was 43.89 ng/ml. Treated with HHT 10 ng/ml for 48 h, K562 cell apoptosis significantly increased, cell cycle was blocked at G0/G1, the expression level of BCL-2 and NF-κB proteins was lower than that in control group (P < 0.05). It is concluded that HHT may inhibit the proliferation of K562 cells, and down-regulating expression levels of BCL-2 and NF-κB may be one of its anti-CML mechanisms.
