Effect of SUV39H1 siRNA silence on apoptosis and proliferation of acute myelogenous leukemia KG-1 cell line.
10.7534/j.issn.1009-2137.2013.01.018
- Author:
Xu-Dong MA
1
;
Ting ZHAO
;
Yi-Qun HUANG
Author Information
1. Department of Hematology, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, Fujian Province, China. xudongma05@yahoo.com
- Publication Type:Journal Article
- MeSH:
Apoptosis;
genetics;
Caspase 3;
metabolism;
Caspase 9;
metabolism;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Methyltransferases;
genetics;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Proto-Oncogene Proteins c-myc;
metabolism;
RNA Interference;
RNA, Small Interfering;
genetics;
Repressor Proteins;
genetics
- From:
Journal of Experimental Hematology
2013;21(1):82-86
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effects of SUV39H1 siRNA on proliferation and apoptosis of acute myelogenous leukemia KG-1 cell line. The small interfering RNA (siRNA) targeting SUV39H1 gene was designed and transfected into KG-1 cells by Lipofectamine(TM) 2000. Cell growth affected by SUV39H1 siRNA was determined by MTS method. Cell apoptosis was measured by flow cytometry. The expressions of P15 and anti-apoptosis protein such as BCL-2, procaspase-9, procaspase-3 and C-MYC were detected by Western blot. The results indicated that siRNA targeting SUV39H1 inhibited proliferation of KG-1 cells. Proliferated rates were (76.43 ± 1.98)%, (51.31 ± 1.84)%, (37.31 ± 1.61)%, (18.94 ± 3.22)% respectively after transfection with SUV39H1 siRNA at 30, 60, 120, 240 nmol/L for 48 h, while P15 expression was upregulated. Apoptotic cells significantly increased, apoptotic rates were (40.2 ± 5.1)%, (56.8 ± 4.8)%, (71.6 ± 5.6)% respectively after transfection with siRNA targeting SUV39H1 at 30, 60, 120 nmol/L (P < 0.05). The protein expression of BCL-2, procaspase-9, procaspase-3, C-MYC was downregulated after transfection. It is concluded that the siRNA targeting SUV39H1 inhibits cell growth and induces cell apoptosis of KG-1 cell line, which may be a new therapeutic target in human leukemia.
