Analysis of influence factors on G-CSF-mobilized hematopoietic stem cells of 191 healthy donors in a mono center.
10.7534/j.issn.1009-2137.2013.01.034
- Author:
Qian WANG
1
;
Lei YUAN
;
Hong-Hua LI
;
Yu ZHAO
;
Wen-Rong HUANG
;
Jian BO
;
Shu-Hong WANG
;
Hai-Yan ZHU
;
Li-Ping DOU
;
Quan-Shun WANG
;
Li YU
;
Yu JING
Author Information
1. Department of Hematology, Chinese PLA General Hospital, Beijing, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Child;
Factor Analysis, Statistical;
Female;
Granulocyte Colony-Stimulating Factor;
pharmacology;
Hematopoietic Stem Cell Mobilization;
methods;
Humans;
Male;
Middle Aged;
Peripheral Blood Stem Cell Transplantation;
methods;
Tissue Donors;
Young Adult
- From:
Journal of Experimental Hematology
2013;21(1):169-172
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the factors influencing mobilization efficiency of peripheral hematopoietic stem cells with granulocyte colony stimulating factor (G-CSF) and their impact on healthy donors. 181 donors were mobilized subcutaneously with G-CSF at 5 - 10 µg/(kg·d), and 10 donors were mobilized with G-CSF at 3.3 - 4.9 µg/(kg·d), once 12 h, for 4 - 5 d. Peripheral blood mononuclear cell (MNC) and CD34(+) cell counts were analyzed by flow cytometry. Mobilization-related side effects were also monitored. The results showed that white blood cell counts increased by 6 times averaged after mobilization (P < 0.01). The platelet count obviously decreased (P < 0.01), while the hemoglobin level did not show significant difference. No significant differences were observed in MNC and CD34(+) cell counts between those subjects harvested on the 4th and 5th day after mobilization. Male donors were superior to female ones in cell harvest (P < 0.01). Donor body weight played positive role in cell yield, while impact of age on harvest was not remarkable. Neither MNC nor CD34(+) cell count showed a linear relationship with G-CSF dose. Only slight side effects were observed on the donors in this study. It is concluded that mobilization with G-CSF is sufficient in healthy donors without remarkable side effects.
