Expression of transcription factors T-bet and GATA-3 in peripheral blood mononuclear cells from patients with systemic lupus erythematosus.
- Author:
Xue-biao PENG
1
;
Yue DENG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Case-Control Studies; Child; Female; GATA3 Transcription Factor; genetics; metabolism; Humans; Leukocytes, Mononuclear; metabolism; Lupus Erythematosus, Systemic; blood; Male; Middle Aged; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; T-Box Domain Proteins; genetics; metabolism; Young Adult
- From: Journal of Southern Medical University 2009;29(10):2138-2139
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of transcription factors T-bet and GATA-3 in the pathogenesis of systemic lupus erythematosus (SLE).
METHODSThe expression of T-bet and GATA-3 mRNA in the peripheral blood mononuclear cells (PBMCs) of 60 patients with SLE and 20 normal control subjects were detected by reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTSCompared with the normal controls, T-bet mRNA expression decreased whereas GATA-3 mRNA expression increased significantly in patients with SLE, active SLE and inactive SLE.
CONCLUSIONSThe high expression of GATA-3 promotes the immature T cells to differentiate into Th2 cells, induces Th2 cells to secrete the cytokines IL-6 and IL-10, which, along with the low expression of Th1 as a result of T-bet expression inhibition, causes B cell activation to produce a large quantity of autoantibodies, leading finally to multiple organ injuries. Effective regulation of GATA-3 expression may provide a new clue for the treatment of SLE.
