Construction and functional identification of red fluorescent protein reporter plasmid for human catalase gene.
- Author:
Qi YAO
1
;
Shao HUANG
;
Ya-Wei LIU
;
Jing-Hua LIU
;
Yong JIANG
Author Information
- Publication Type:Journal Article
- MeSH: 3T3 Cells; Animals; Catalase; genetics; Gene Expression; Genes, Reporter; genetics; Genetic Vectors; biosynthesis; genetics; Humans; Hydrogen Peroxide; pharmacology; Luminescent Proteins; biosynthesis; genetics; Mice; Plasmids; genetics; Promoter Regions, Genetic; Transfection
- From: Journal of Southern Medical University 2009;29(11):2149-2151
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct a red fluorescent protein reporter gene driven by human catalase gene promoter.
METHODSThe red fluorescent protein reporter gene plasmid pDsRed-CATp containing human catalase gene promoter was constructed by gene recombination technique. The plasmid was transiently transfected into NIH/3T3 cells to observe their response to H(2)O(2) stimulation.
RESULTSThe plasmid was constructed correctly as verified by double enzyme digestion and sequence analysis. The plasmid was lowly expressed in resting NIH/3T3 cells, but the expression level increased obviously after stimulation by H(2)O(2). CONCLSIONS: A red fluorescent protein reporter gene plasmid driven by human catalase gene promoter has been constructed successfully with a sensitive response to H(2)O(2) stimulation. This system provides a convenient tool for the study of the regulatory mechanism of catalase gene expression.
