Effect of cytotoxin interleukin-2-pseudomonas exotoxin 66 on corneal allograft rejection in mice.
- Author:
Jing WU
1
;
Jian YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bacterial Proteins; therapeutic use; Corneal Transplantation; adverse effects; Cytotoxins; therapeutic use; Exotoxins; therapeutic use; Female; Graft Rejection; drug therapy; Graft Survival; drug effects; Immunosuppressive Agents; therapeutic use; Interleukin-2; therapeutic use; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Random Allocation; Recombinant Fusion Proteins; therapeutic use
- From: Journal of Southern Medical University 2009;29(11):2152-2155
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the immunosuppressive effect of the interleukin-2-pseudomonas exotoxin 66(IL-2-PE66) on murine corneal allograft rejection.
METHODSThirty-six recipient female BALB/c mice received corneal allografts from C57BL/6 mice and were divided randomly into treatment and control groups. The condition of the grafts was observed twice a week. On days 10, 15, 25 and 35 after the transplantation, the operated eyes were removed for pathological examinations. Peripheral blood samples were also collected for analysis of T cell subsets and T lymphocyte colony forming unit (T-CFU) assay.
RESULTSThe survival time of corneal allograft averaged 15.8-/+2.1 days in the control group and 31.2-/+2.9 days in the treatment group. The CD(4)(+)/ CD(8)(+)/ of the T cell subsets 15 days after the operation was 1.26-/+0.23 in the treatment group and 2.01-/+0.23 in the control group, with T-CFU of 201-/+18.2 and 286-/+16.8, respectively.
CONCLUSIONIL-2-PE66 can delay the development of corneal graft rejection, significantly reduce the percentage of T helper cells, and weaken the aggregation of the peripheral T cells.
