Expression of human triggering receptor expressed on myeloid cells 1 in peripheral blood mononuclear cells of patients with acute obstructive suppurative cholangitis.
- Author:
Yong MIAO
1
;
Zuo-Jin LIU
;
Jian-Ping GONG
;
Si-Dong WEI
;
Fa-Liang XU
;
Zhen-Zhen CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Adult; Aged; Aged, 80 and over; Biomarkers; blood; Case-Control Studies; Cholangitis; blood; complications; Enzyme-Linked Immunosorbent Assay; Female; Humans; Leukocytes, Mononuclear; metabolism; Male; Membrane Glycoproteins; genetics; metabolism; Middle Aged; RNA, Messenger; genetics; metabolism; Receptors, Immunologic; genetics; metabolism; Sepsis; blood; etiology; Triggering Receptor Expressed on Myeloid Cells-1
- From: Journal of Southern Medical University 2009;29(11):2179-2181
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression pattern of human triggering receptor expressed on myeloid cells 1 (TREM-1) mRNA in peripheral blood mononuclear cells and its clinical significance in acute obstructive suppurative cholangitis (AOSC).
METHODSPeripheral blood mononuclear cells were collected from 36 patients with AOSC and 40 healthy adults. TREM-1 mRNA was determined by semi-quantitative RT-PCR, and TREM-1 protein by immunocytochemistry. Enzyme-linked immunosorbent assay (TNF-alpha) was used to detect the level of tumor necrosis factor-alpha (TNF-alpha), and immunoturbidimetry employed to detect C reactive protein.
RESULTSThe expression of TREM-1 mRNA relative to beta-actin was 1.007-/+0.252 in patients with AOSC, significantly higher than that in the healthy adults (0.457-/+0.053, P<0.05). The two groups also showed significantly different TREM protein expression (P<0.01). The AOSC patients exhibited significantly higher levels of TNF-alpha and C reactive protein than the healthy adults (P<0.01).
CONCLUSIONThe expression of human TREM-1 in peripheral blood mononuclear cells is up-regulated obviously in early stage of AOSC, probably suggesting an important role of TREM-1 in the development of AOSC.
