Comparison of DNA-dependent protein kinase catalytic subunit expression in two lung adenocarcinoma cell lines with different radiosensitivity.
- Author:
Wei-Jian CEN
1
;
Yi PAN
;
Wei-Xiong LI
;
Su-Qing YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; enzymology; pathology; Calcium-Binding Proteins; genetics; metabolism; Cell Line, Tumor; Humans; Lung Neoplasms; enzymology; pathology; Radiation Tolerance
- From: Journal of Southern Medical University 2009;29(11):2241-2243
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate DNA-dependent protein kinase catalytic subunit (DNA-PKcs) content and activity in lung adenocarcinoma cell lines and its correlation with radiosensitivity.
METHODSThe content and activity of DNA-PKcs were analyzed in two lung adenocarcinoma cell lines A549 and H1299 by Western blotting and the Signa TECT DNA-PK assay kit. The dose-survival relationship for two cell lines was analyzed using clonogenic formation assay.
RESULTSA549 was more radiosensitive than H1299. The survival fractions at 2 Gy (SF2) were 0.7412 in A549 cell line and 0.2473 in H1299 cell line. The content of DNA-PKcs was significantly higher in A549 cells than in H1299 cells (t=10.37, P<0.001). The integrated optical densities were 3.29-/+0.44 in A549 cells and 0.50-/+0.17 in H1299 cells. DNA-PKcs activities in A549 and H1299 cells were 8.29-/+1.37 and 2.47-/+1.09, respectively, showing a significant difference between them (t=5.76, P=0.005).
CONCLUSIONDNA-PKcs is an important factor to affect the radiosensitivity of lung adenocarcinoma cell lines.
