Absorption characteristics of ginsenoside Rb2 in Caco-2 cell monolayer.
- Author:
Jie ZHAO
1
;
Cai-hua YANG
;
Ming HU
;
Zhong-qiu LIU
Author Information
- Publication Type:Journal Article
- MeSH: Caco-2 Cells; Gas Chromatography-Mass Spectrometry; methods; Ginsenosides; pharmacokinetics; Humans; Intestinal Absorption
- From: Journal of Southern Medical University 2009;29(12):2387-2390
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the concentration of ginsenoside Rb(2) and study the absorption characteristics of ginsenoside Rb(2) in Caco-2 cell monolayer.
METHODSLC-MS-MS was used to determine the concentration of ginsenoside Rb(2), and the apparent permeability coefficient (P(app)) of ginsenoside Rb(2) was calculated.
RESULTSP(app(AP-BL)) was 3.27 x 10(-7) cm.s(-1), P(app(BL-AP)) was 3.16 x 10(-6) cm.s(-1), and the efflux ratio (P(app(BL-AP))/P(app(AP-BL))) was 9.63.
CONCLUSIONThe absorption characteristics of ginsenoside Rb(2) in Caco-2 cell model have been demonstrated.
