A rapid colorimetric assay for viability of osteosarcoma cells after chemotherapy.
- Author:
Qiang CHEN
1
;
Jin-hua ZHANG
;
Jian-ting CHEN
;
Zhan-jun SHI
;
Yong-jian DENG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; therapeutic use; Bone Neoplasms; drug therapy; pathology; Cell Survival; drug effects; Colorimetry; methods; Formazans; Humans; Osteosarcoma; drug therapy; pathology; Sensitivity and Specificity; Tetrazolium Salts
- From: Journal of Southern Medical University 2009;29(12):2490-2491
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo introduce a rapid colorimetric method for assessing the viability of osteosarcoma cells after chemotherapy.
METHODSColorimetric assay and automatic microplate scanning spectrophotometer were used for assaying the viability of osteosarcoma cells.
RESULTSClose correlation was found between the absorbance at 570 nm of the formazan products and the number of viable osteosarcoma cells.
CONCLUSIONAn effective, sensitive and convenient colorimetric assay has been established to assess the survival of osteosarcoma cells following chemotherapy.
