Dynamic changes of serum MCP-1 and MIP-2 chemokines after renal transplantation in rats.
- Author:
Hao YAO
1
;
Jun-jie YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chemokine CCL2; blood; Chemokine CXCL2; blood; Cyclosporine; pharmacology; Graft Rejection; blood; drug therapy; Kidney Transplantation; adverse effects; Male; Postoperative Period; Rats; Rats, Sprague-Dawley; Rats, Wistar; Reperfusion Injury; blood
- From: Journal of Southern Medical University 2009;29(12):2512-2516
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes in serum levels of chemokine MCP-1 and MIP-2 after renal transplantation in rats and the influence of Cyclosporin A (CsA) on their levels.
METHODSThree groups of rats, namely untreated group, CsA group and isograft group underwent the renal transplantation, and the serum MCP-1 and MIP-2 levels of the recipients were tested using enzyme-linked immunosorbent assay (ELISA).
RESULTSThe serum MCP-1 level peaked 6 hours after the operation and also in critical stages of acute rejection. The first peak of MCP-1 was postponed by the application of CsA, which did not affect the peak level (P>0.05). The second peak of MCP-1 did not occur in CsA-treated group. MIP-2 concentration also peaked at 6 h after the operation in all the groups. The second peak of MIP-2, which was lower than the first one (P<0.05), occurred 9 days after the transplantation.
CONCLUSIONMCP-1 and MIP-2 in involved not only in the ischemia-reperfusion injury but also in severe acute rejection. CsA has no significant effect on serum levels of MCP-1 and MIP-2 following renal transplantation in rats.
