Study on the molecular epidemiology of beta-lactamase TEM gene in isolated Streptococcus pneumoniae.
- Author:
Yun-fang DING
1
;
Jian-hua ZHANG
;
Zu-huang MI
;
Ling QIN
;
Yun-zhen TAO
;
Xiao QI
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; China; epidemiology; Drug Resistance, Bacterial; genetics; Female; Genes, Bacterial; genetics; Humans; Male; Molecular Epidemiology; Molecular Sequence Data; Pneumonia, Bacterial; epidemiology; genetics; microbiology; Point Mutation; Streptococcus pneumoniae; enzymology; genetics; beta-Lactam Resistance; beta-Lactamases; genetics
- From: Chinese Journal of Epidemiology 2004;25(11):970-972
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the beta-lactamase TEM gene of isolated Streptococcus pneumoniae (Sp) in Suzhou area.
METHODSTwenty-three strains of Sp were collected from respiratory tract secretions of children with respiratory diseases in Nov 2002 to Apr 2003 at Children's Hospital of Suzhou University (reference strain ATCC49619) to build TEM polymerase chain reaction (PCR) system (reference strain E. coli. 9-j53R1 with TEM gene) TEM gene of 23 strains was detected to comparo the sequences with published TEM gene sequences in GenBank for analyzing TEM gene model.
RESULTSTwenty-one strains had TEM gene with a positive rate of 91.3% (21/23). TEM-129 gene were confirmed from No.17 (SR017, penicillin resistance) TEM sequence. New discovered TEM-129 sequence had a modification (ATG[M]-->ATA[I]) at No.182 code and published (GenBank: www.ncbi.nlm.nih.gov/nucleotide, AY452662). TEM-1 genes were confirmed from other TEM sequences. New discovered TEM-1 gene of isolated Sp had been published (GenBank: www.ncbi.nlm.nih.gov/nucleotide, AY392531) too.
CONCLUSIONIsolated Sp had TEM gene (TEM-129, EM-1 genotype) with a positive rate of 91.3%. The result enriched the understanding of isolated Sp with penicillin resistance.
