Genetic association of apoE and apoCI gene polymorphisms with coronary heart disease.
- Author:
Chun-Hong WANG
1
;
Xin ZHOU
;
Guang-di ZHOU
;
Xiao-dong TAN
;
Ding-fen HAN
;
Fang ZHENG
;
Fang LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Alleles; Apolipoproteins C; genetics; Apolipoproteins E; genetics; China; epidemiology; Coronary Disease; blood; epidemiology; genetics; Female; Genotype; Haplotypes; Humans; Male; Middle Aged; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Risk Factors
- From: Chinese Journal of Epidemiology 2004;25(11):982-985
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the genetic association of apolipoprotein (apo) E and apoCI gene polymorphisms with coronary heart disease (CHD) in China.
METHODSapoE genotypes were identified by multiplex amplification refractory mutation system (multi-ARMS) and the apoCI promoter polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 186 cases with CHD (age: 65.0 +/- 10.5 years) and 350 controls (age: 63.6 +/- 8.3 years). The haplotype frequencies were estimated.
RESULTSThe frequencies of apoE E4/3 genotype (26.9%) and epsilon4 (14.5%) in CHD group were significantly higher than that in the control group (12.6%, 7.0%), P <0.05. The significant difference was also found for the apoCI locus and the CHD group showed higher rate of both for the H2 allele and genotypes, carrying this allele. Estimation of the haplotype frequencies indicated that the association between the apoE-CI haplotype and CHD was significantly strong. The apoE-epsilon4/apoCI-H2 was estimated to be responsible for 9.86% of CHD.
CONCLUSIONWhen the subjects carrying both epsilon4 and H2 alleles, they would have higher risk of suffering from CHD than controls.
