Development of bone marrow mesenchymal stem cell culture in vitro.

Li Zhang; Li-pan Peng; Nan WU; Le-ping LI

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Development of bone marrow mesenchymal stem cell culture in vitro.

Author: Li ZHANG ¹ ; Li-Pan PENG ; Nan WU ; Le-Ping LI
Author Information

1. Department of Gastrointestinal Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, China. pzzl@sina.com
Publication Type:Journal Article
MeSH: Animals; Bone Marrow Cells; cytology; Cell Culture Techniques; methods; Cells, Cultured; Humans; Mesenchymal Stromal Cells; cytology
From: Chinese Medical Journal 2012;125(9):1650-1655
CountryChina
Language:English
Abstract:
OBJECTIVETo review the in vitro development of bone marrow mesenchymal stem cells culture (BM-MSC).
DATA SOURCESThe data cited in this review were mainly obtained from articles listed in Medline and PubMed. The search terms were "bone marrow mesenchymal stem cell" and "cell culture".
STUDY SELECTIONArticles regarding the in vitro development of BM-MSCs culture, as well as the challenge of optimizing cell culture environment in two-dimensional (2D) vs. 3D.
RESULTSImproving the culture conditions increases the proliferation and reduces the differentiation. Optimal values for many culture parameters remain to be identified. Expansion of BM-MSCs under defined conditions remains challenging, including the development of optimal culture conditions for BMSC and large-volume production systems.
CONCLUSIONSExpansion of BM-MSCs under defined conditions remains challenges, including the development of optimal culture conditions for BMSC and scale-up to large-volume production systems. Optimal values for many culture parameters remain to be identified.