Anti-cancer activity of Zuojinwan in vivo and influence to tumor markers in mice transplanted with sarcoma 180.
- Author:
Xiao-Na WANG
1
;
Qin ZHOU
;
Xu HAN
;
Li-Na XU
;
Jin-Yong PENG
Author Information
- Publication Type:Journal Article
- MeSH: Acid Phosphatase; blood; Alkaline Phosphatase; blood; Animals; Antineoplastic Agents; pharmacology; Biomarkers, Tumor; metabolism; Coptis; chemistry; Creatine Kinase; blood; Drugs, Chinese Herbal; pharmacology; Evodia; chemistry; Female; Fructose-Bisphosphate Aldolase; blood; L-Lactate Dehydrogenase; blood; Male; Mice; Neoplasm Transplantation; Random Allocation; Sarcoma 180; blood; drug therapy; pathology
- From: China Journal of Chinese Materia Medica 2008;33(19):2230-2234
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the anticancer action of Zuojinwan in mice transplanted with sarcoma 180 in vivo, and detect the activities of five kinds of tumor markers (TM) including acid phosphotase (ACP), alkaline phosphotase (AKP), creatine kinase (CK), aldolase (ALD) and lactate dehydrogenase (LDH) in serum compared with Coptis chinensis and Evodia rutaecarpa.
METHODThe transplanted S180 tumor mice model was established, and the mice were divided randomly five groups. The extract of Zuojinwan (850.8 mg kg(-1)), C. chinensis (729.2 mg kg(-1)) and E. rutaecarpa (121.6 mg kg(-1)) were administrated, respectively for 10 d. Then, the changes of body weight, spleen index of mice, the inhibition rates of tumor, and the increase of life span (ILS) were all tested. In addition, the activities of ACP, AKP, CK, ALD and LDH on different test groups were also determined.
RESULTZuojinwan could inhibit the S180 tumor growth significantly with the inhibition rate of 50.54% and the ILS of mice reached to 64.91%. Meanwhile, the activities of ACP (126.72 +/- 11.16) U 100 mL(-1) and AKP (67.27 +/- 13.49) U 100 mL(-1) were increased, and the activities of CK (20.65 +/- 4.28) U mL(-1), ALD (319.13 +/- 53.87) U L(-1) and LDH (1,029.04 +/- 468.56) U L(-1) were decreased significantly by Zuojinwan treated group compared with C. chinensis and E. rutaecarpa treated groups (P<0.01).
CONCLUSIONIn the prescription of Zuojinwan, the enhancement of compatibility of anticancer activity was observed by the interaction of C. chinensis and E. rutaecarpa. The mechanism might be in according with to influence the activities of the five kinds of tumor markers (TM) in mice serum.
