Effect of matrine and oxymatrine on proliferation and expression of Stat3 and Stat5 in SMMC-7721 cell line.
- Author:
Yan-Min ZHENG
1
;
Xuan LI
;
Hong-Yan ZHAO
;
Jun-Yan ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; pharmacology; Apoptosis; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Drugs, Chinese Herbal; pharmacology; Gene Expression Regulation; drug effects; Humans; Quinolizines; pharmacology; Reverse Transcriptase Polymerase Chain Reaction; STAT3 Transcription Factor; genetics; STAT5 Transcription Factor; genetics
- From: China Journal of Chinese Materia Medica 2008;33(19):2234-2237
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of matrine and oxymatrine on proliferation and the expression of Stat3, Stat5 mRNA in SMMC-7721 cell line.
METHODTreated with matrine and oxymatrine, the inhibitory effect on SMMC-7721 cell proliferation was detected by MTT, double fluorescence labeling was applied to measure the apotosis ratios of SMMC-7721cells, the expression of Stat3 and Stat5 mRNA in SMMC-7721 cell line were assessed with RT-PCR.
RESULTMatrine and oxymatrine could inhibit the proliferation and induce the apoptosis of SMMC-7721 cells and it was time and dose dependent, the expression of Stat3 and Stat5 mRNA in SMMC-7721 cell with matrine and oxymatrine were significantly lower than those in control group (P<0.05 or P<0.01). Compared with the same dose of matrine and oxymatrine, matrine showed stronger effect on cell proliferation, apoptosis, and Stat3 and Stat5 mRNA (P<0.05 or P<0.01).
CONCLUSIONMatrine and oxymatrine inhibited the proliferation and induced the of SMMC-7721 cells significantly, the mechanism of which might be related to the down-regulation of stat3 and stat5 mRNA and inhibition of the signaling transduction pathway.
