Analysis of genetic diversity of wild Rehmannia glutinosa by using RAPD and ISSR markers.
- Author:
Yan WANG
1
;
Xian-en LI
;
Xue-dong LI
;
Jian-jun QI
;
Peng SUN
;
Li-li ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Genetic Variation; genetics; Phylogeny; Random Amplified Polymorphic DNA Technique; methods; Rehmannia; classification; genetics
- From: China Journal of Chinese Materia Medica 2008;33(22):2591-2595
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the genetic diversity of wild Rehmannia glutinosa and evaluate and compare random amplified polymorphic DNA (RAPD) and inter sample sequence repeat (ISSR) for analysis of R. glutinosa accessions.
METHODTwo molecular markers, RAPD and ISSR were used for analyzing 55 wild R. glutinosa accessions.
RESULTAverage 16.00 and 19.08 bands were amplified by RAPD primers and ISSR primers respectively, and the percentage of polymorphic bands were 89.58% and 94.32% respectively; Fifty-five R. glutinosa accessions categorized into 7 clusters were identified by unweighted pair-group method, arithmetic average (UPGMA) method.
CONCLUSIONA high level of genetic diversity of wild Rehmannia glutinosa was displayed at DNA level, and genetic diversity coefficient of R. glutinosa from different production areas was 0.63-0.98, and ISSR marker can detect higher genetic diversity of R. glutinosa germplasms than RAPD marker.
