Study on bulblet induction of Fritillaria anhuiensis in vitro.
- Author:
Jian-ping XUE
1
;
Ai-min ZHANG
;
Mao-lin GENG
;
Lin MA
Author Information
- Publication Type:Journal Article
- MeSH: Fritillaria; drug effects; growth & development; Plant Stems; drug effects; growth & development; Plants, Medicinal; drug effects; growth & development; Salicylic Acid; pharmacology; Sucrose; pharmacology; Temperature
- From: China Journal of Chinese Materia Medica 2008;33(22):2603-2606
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the optimal condition for bulb induction of Fritillaria anhuiensis in vitro.
METHODEffects of sucrose, salicylic acid, active carbon and 5 degrees C pre-incubation on bulb formation in vitro were investigated by adopting the number of bulb and fresh weight as indexes.
RESULTThe number of bulb and fresh weight in medium added with 50 g x L(-1) sucrose were significantly higher than those with other treatments. The medium with different concentrations of salicylic acid showed no significant promotion on callus differentiation. However, 5 g x L(-1) active carbon (AC) treatment was better than other treatments. Meanwhile, a large number of plantlets formed after 5 degrees C pre-incubation for 30-40 days was suitable for bulb formation and growth.
CONCLUSIONBulblet formation was optimal in MS + KT 2 mg x L(-1) + NAA 2 mg x L(-1) + 50 g x L(-1) sucrose +5 g x L(-1) AC after callus pre-incucation at 5 degrees 1 for 30-40 days.
