Study on stability of baicalin and baicalein in Rat plasma by HPLC-ECD.
- Author:
Qi WANG
1
;
Yu-jie ZHANG
;
Wei-feng LI
;
Jie YANG
;
Hao JIANG
;
Wan-jing SUN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chromatography, High Pressure Liquid; methods; Flavanones; blood; pharmacokinetics; Flavonoids; blood; pharmacokinetics; Hydrogen-Ion Concentration; Male; Rats; Rats, Sprague-Dawley; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2008;33(22):2675-2678
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the stability of baicalin and baicalein in buffered aqueous solutions and mouse plasma in order to discuss the degradation mechanism and the method of disposing samples.
METHODHPLC-ECD was used to determine baicalin and baicalein in different conditions, and the disposing samples methods were selected to stabilize them.
RESULTBaicalin and baicalein in the buffered aqueous solution at pH 7.4 was more stable than that in the mouse plasma. After adding Vit C in the buffered aqueous solution, the stability of baicalin and baicalein could be improved obviously and their remained percents were 102% and 100%. The stability of baicalin and baicalein could be enhanced distinctly in the mouse plasma only when adding Vit C and 1 mol x L(-1) HCl, and furthermore their remained percentage was up to 98% and 103%.
CONCLUSIONThe stability of baicalin and baicalein is related to the oxidation and enzyme degradation and it can be improved by adding Vit C and 1 mol x L(-1) HCl in the solution.
