Isolation-preparation and determination of chromones from Saposhnikovia divaricata.
- Author:
Cun ZHANG
1
;
Yong-qing XIAO
;
Li LI
;
Zhen PANG
;
Gui-liu LI
Author Information
- Publication Type:Journal Article
- MeSH: Apiaceae; chemistry; Chromones; analysis; isolation & purification; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2008;33(23):2761-2764
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the contents of chromones in the different isolation and purification procedures from Saposhnikovia divaricata.
METHODThe separation of six chromones was determined simultaneously by HPLC on a Kromasil C18 column at 35 degrees C with the methanol-0.1% phosphoric acid in gradient elution as the mobile phrase. The detection wavelength was set at 254 nm and the flow rate was 1.0 mL x min(-1).
RESULTAll the obtained correlation coefficient of the six components was over 0.9995 and the average recoveries were not less than 97% (RSD <5%), respectively.
CONCLUSIONThe method is simple, accurate with good reproducibility. Results showed the better preparation procedure is water-decoction and ethanol-precipitation with resin separation and silica gel purification.