Studies on anticoagulant constituents in dried Whitmania pigra.
- Author:
Shan ZHONG
1
;
De-po YANG
;
Zheng CUI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anticoagulants; analysis; chemistry; isolation & purification; Leeches; chemistry; Molecular Weight
- From: China Journal of Chinese Materia Medica 2008;33(23):2781-2784
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the anticoagulant constituents in dried leech (Whitmania pigra Whitman).
METHODThe plasma recalcification time (PRT) as index, the constituents with anticoagulant activity were isolated and purified by anion-exchange chromatography on Sephadex DEAE A-50, gel permeation chromatography on Sephadex G-25 and Sephadex LH-20 columns, and then reversed phase high-performance liquid chromatography successively.
RESULTThree anticoagulant polypeptides were isolated and purified. Compounds 1 and 2 can be translated each other in natural conditions, and their molecular weights are 7100 and 5531, respectively. Compound 3 was identified as a pure polypeptide by HPLC and SDS-PAGE, and its molecular weight was determined as 8 608 by MALDI-TOF-MS. The amino acid composition of compound 3 was also determined.
CONCLUSIONCompound 3 was inferred to be a novel anticoagulant, and named whitmanin.
