Determination of anthraquinone in Semen Cassiae from different regions by HPLC.
- Author:
Yi ZHANG
1
;
Xiao-ping HUANG
;
Dai-qun WENG
;
Da-jian YANG
Author Information
- Publication Type:Journal Article
- MeSH: Anthraquinones; analysis; Cassia; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; classification; isolation & purification
- From: China Journal of Chinese Materia Medica 2008;33(23):2797-2799
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the content of 7 anthraquinones in Semen Cassiae.
METHODA HPLC method was developed, with Inertsil ODS-3 column, acetonitrile and 0.1% H3PO4 solution as mobile phases in gradient elution. The detection wavelength wasset at 278 nm, and the flow rate was 0.8 mL x min(-1).
RESULTRecoveries of all 7 anthraquinones were between 95%-105%. The content of the anthraquinones in crude drug produced in different habitation were different.
CONCLUSIONThe method is convenient and accurate, which provides the foundation for the research of Semen Cassiae.
