Determination of eleutheroside B in antifatigue fraction of Acanthopanax senticosus by HPLC.
- Author:
Chen LI
1
;
Xiao-yan WANG
;
Xu-wei HU
;
Hai-tao FANG
;
Shan-yi QIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; isolation & purification; Eleutherococcus; chemistry; Fatigue; drug therapy; physiopathology; Glucosides; analysis; Humans; Male; Mice; Phenylpropionates; analysis; Swimming
- From: China Journal of Chinese Materia Medica 2008;33(23):2800-2802
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for the determination of eleutheroside B in As1 (the extract fraction from Acanthopanax senticosus, which has a good effect of antifatigue).
METHODThe antifatigue effect of Asl was evaluated by mice burden swimming testing. One compound was isolated by silica gel, Sephadex LH-20 column chromatography and preparative high performance liquid chromatography. The structure was identified by physicochemical properties and spectral evidences. Eleutheroside B in Asl was determined by HPLC. Chromatographic conditions included Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) and the mobile phase consisting of a mixture of acetonitrile-water-ethanoic acid (10: 90: 0.01). The UV detection wavelength was set at 344 nm.
RESULTAs1 showed an excellent antifatigue activity; One compound was isolated from As1 and its structure was identified as Eleutheroside B; The calibration cure was linear in the range of 0.104-20.8 microg (r = 0.9999), the average recovery was 97.68%, RSD 1.4% (n=6).
CONCLUSIONThis HPLC method is simple,accurate and reliable.
