Research for dependablity of administration of platycodi radix in Tianwang Buxinwan decoction with change of brain inhibitive neurotransmitte in rats by microdialysis.
- Author:
Ping LIU
1
;
Xin-rong HE
;
Wen-bin ZHOU
;
Ran-ran SHEN
;
Fan FENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; drug effects; metabolism; Brain Chemistry; Drugs, Chinese Herbal; administration & dosage; Male; Microdialysis; Neurotransmitter Agents; antagonists & inhibitors; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2008;33(23):2830-2833
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of Tianwang Buxinwan decoction on the contents of amino acids neurotransmitters in corpus striatum of rats to implicate the mechanism of Tianwang Buxinwan promoting and Improving sleeping.
METHODContents of two amino acids neurotransmitters in corpus striatum of rats were prepared by microdialysis technology and were determined by HPLC which involved pre-column derivation with orthophthaladehyde, recersed-phase gradient elution and fluorescence detection.
RESULTIn the experimental separation condition, Tianwang Buxinwan seemed do not influence three kinds of contents of amino acids neurotransmiters (glutamic acid, glycin, aspartic acid), but TBW seemed increase the content of gamma-GABA in corpus striatum of rats.
CONCLUSIONThe effects of Tianwang Buxinwan to relieve uneasiness may relate with the inhibitory amino acids gamma-GABA. Tianwang Buxinwan may promote increasing the content of gamma-GABA. This discovery may be helpful for the deep study of related mechanism of Tianwang Buxinwan.
