Determination of zizybeoside II of Ziziphus jujuba by HPLC.
- Author:
Ji-Wei NIU
1
;
Qi-Wei ZHANG
;
Mu-Xin GONG
;
Li-Hua YAN
;
Jing-Jing ZHU
;
Zhi-Min WANG
;
Qin-Fan LI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; isolation & purification; Saponins; analysis; Ziziphus; chemistry
- From: China Journal of Chinese Materia Medica 2008;33(24):2935-2937
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a quantitative method for determination of zizybeoside II in Ziziphus jujuba.
METHODThe samples were separated at 30 degrees C on a Zorbax SB-C18 column eluted with methanol-water (20 : 80) as the mobile phase. Flow rate was set at 1.0 mL x min(-1) and the detection wavelength was set at 210 nm.
RESULTThe calibration curve was linear within the range from 0.046 to 0.582 microg (r = 0.999 9) and the average recovery was 97.2%. 12 batches of the crude drugs purchased from different areas were determined and the contents of zizybeoside II in Fructus Jujubae were fluctuated from 0.013% to 0.041%.
CONCLUSIONThe method is simple, repeatable and could be used for the quality control of Z. jujuba.
