Comparison of Protein Expression in Normal Myometrium and Uterine Leiomyoma Using Two-Dimensional Gel Electrophoresis in Korean Women.
- Author:
Seung Ku LEE
1
;
Su Mi BAE
;
Ko Woon KIM
;
Min Sook KIM
;
Eun Kyung PARK
;
Yong Wook KIM
;
Duck Young RO
;
Joon Mo LEE
;
Seung Eun NAMKOONG
;
Chong Kook KIM
;
Woong Shick AHN
Author Information
1. Cancer Research Center, Catholic Research Institutes of Medical Science, College of Medicine, The Catholic University of Korea.
- Publication Type:Original Article
- Keywords:
Cancer;
Leiomyoma;
Myometrium;
Two-dimensional gel electrophoresis (2-DE);
Matrix assisted laser desorption/ionization-time of flighting (MALDI-TOF)
- MeSH:
Actins;
Adenosine Triphosphate;
alpha Catenin;
Animals;
Annexin A4;
Carrier Proteins;
Cofilin 2;
Databases, Protein;
Electrophoresis, Gel, Two-Dimensional*;
Electrophoresis, Polyacrylamide Gel;
Female;
Glutathione Transferase;
Heat-Shock Proteins;
Humans;
Hypoxanthine Phosphoribosyltransferase;
Isoelectric Focusing;
Keratins, Type II;
Leiomyoma*;
Mice;
Myometrium*;
Parkinsonian Disorders;
Peptide Elongation Factor Tu;
Peptide Elongation Factors;
Peroxiredoxins;
Phospholipid Transfer Proteins;
Proteasome Endopeptidase Complex;
Receptors, Tumor Necrosis Factor;
Running;
Serum Amyloid P-Component;
Sodium Dodecyl Sulfate;
Tretinoin
- From:Korean Journal of Obstetrics and Gynecology
2004;47(4):618-626
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: Comparison of protein expression by two-dimensional gel electrophoresis (2-DE) in normal myometrium and uterine leiomyoma in Korean women. METHODS: Normal myometrium and uterine leiomyoma tissues were solubilized with 2-DE buffer and the first dimension of PROTEAN IEF CELL, isoelectric focusing (IEF), was performed using pH4-8 linear IPG strips of 17 cm. And then running 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS- PAGE) and sliver stain. Scanned image analyzed using PDQuest 2-D softwareTM. Protein spot spectrum was identified by assisted laser desorption/ionization-time of fighting (MALDI-TOF) and the protein mass spectrums identification were performed by searching protein databases of Swiss-prot/TrEMBL, Mascot and MS-FIT. RESULTS: In this study, we found 17 up-regulated proteins (phosphate carrier protein, 60 kDa heat shock protein, acidic calcium-independent, glutathione transferase omega, chloride intracellular channel 4, Ras-related protein Rab-11B, phosphatidylinositol transfer protein alpha isoform, type II keratin subunit protein, Cofilin 2 isoform 1, transgelin, ATP carrier protein, alpha-catenin homolog, parkinson disease 2, apo-cellular retinoic acid binding protein II, osteoglycin preproprotein, proteasome activator subunit 1 isoform, Unnamed protein) and 7 down-regulated proteins (Serum amyloid P component, annexin IV, alpha 1 actin precursor, hypoxanthine-guanine phosphoribosyltransferase, tumor necrosis factor receptor superfamily member EDAR precursor, peroxiredoxin 2, translation elongation factor EF-Tu precursor) between myometrium and leiomyoma. CONCLUSION: 2-DE offer total protein expression between normal myometrium and uterine leiomyoma, and searching of differently expressed protein for the diagnostic markers of leiomyoma.
