Pilot-scale production of recombinant plasmid pUDK-HGF.
- Author:
Chunsheng HU
1
;
Yanliang WANG
;
Yuxin LU
;
Xiaochen CHENG
;
Lin LIU
;
Tong ZHANG
;
Qinglin ZHANG
Author Information
1. Beijing Institute of Radiation Medicine, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
DNA, Recombinant;
biosynthesis;
DNA, Superhelical;
isolation & purification;
Escherichia coli;
genetics;
metabolism;
Fermentation;
Genetic Vectors;
genetics;
Hepatocyte Growth Factor;
biosynthesis;
genetics;
Humans;
Pilot Projects;
Plasmids;
isolation & purification
- From:
Chinese Journal of Biotechnology
2011;27(2):247-252
- CountryChina
- Language:Chinese
-
Abstract:
pUDK-HGF, the recombinant plasmid DNA encoding human hepatocyte growth factor (HGF), can treat ischaemic disease. A great quantity of pharmaceutical pUDK-HGF is needed. A pilot-scale production process of pUDK-HGF was established based on a new chromatographic media (plasmidselect), including fermentation, cell harvesting, alkaline lysis, ultrafiltration, RNA removing and buffer exchanging on Sephacryl S-1000, capturing supercoiled plasmid DNA with plasmidselect, and removing the salt with Sepharose 6BFF. The process does not use RNase enzyme and toxic solvents.
